654 Novel immune-related proteins differentially expressed in melanoma

Novel immune-related targets hold promise to improve melanoma treatment and patient survival. Using bioinformatics, we previously identified 21 molecules differentially expressed in metastatic have significant correlation with We shortlisted five candidate that been implicated pathogenesis of other cancer types, however, none has explored melanoma. These are (i) MZB1, a B cell-specific cochaperone. (ii) SAMSN1, negative regulator B-cell activation regulates cell polarization. (iii) NCKAP1L, which controls lymphocyte proliferation, phagocytosis by neutrophils/macrophages. (iv) KLK8, an epidermal protease involved the skin barrier proteolytic cascade. (v) EBI3, functions innate immune responses. In this study, aimed validate expression profile these at protein level clinical tissues along S100, biomarker. performed fluorescent immunostaining human tissue microarray (ME2082c; US Biomax) consisting 208 cores (128 primary melanoma, 64 8 adjacent normal skin) followed multispectral Vectra Imaging coupled inForm software analysis. observed significantly increased (p<0.05) SAMSN1 EBI3 S100-positive tumor cells compared S100-negative cells. Further, MZB1 NCKAP1L levels were skin. KLK8 showed increasing trend tissues. Interestingly, was not changed between quantified using Simple Western A375, G361, HS294T, WM115 melanocytes as well tumors obtained from BrafV600E/PtenNULL mice (n=5) Overall, our study suggest could be potential new